An investigation of methods for enriching trophoblast from maternal blood

Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method.     

A Simulation of Temporal and Spatial Variations in Carbon at Landscape Level: A Case Study for Lake Abitibi Model Forest in Ontario,Canada

Using a case study of the Lake Abitibi Model Forest (LAMF), this study aims to assess the temporal and spatial variability in carbon storage during 1990–2000, and to present a comprehensive estimation of the carbon budget for LAMF's ecosystems. As well, it provided the information needed by local forest managers to develop ecological and carbon-based indicators and monitor the sustainability of forest ecosystems. Temporal and spatial carbon dynamics were simulated at the landscape level using ecosystem model TRIPLEX1.0 and Geographical Information System (GIS). The simulated net primary productivity (NPP) and carbon storage in forest biomass and soil were compared with field data and results from other studies for Canada's boreal forests. The results show that simulated NPP ranged from 3.26 to 3.34 tC ha⁻¹ yr⁻¹ in the 1990s and was consistent with the range measured during the Boreal Ecosystem-Atmosphere Studies (BOREAS) in central Canada. Modeled NPP was also compared with the estimation from remote sensing data. The density of total above-and belowground biomass was 125.3, 111.8, and 106.5 tC ha⁻¹ for black spruce, trembling aspen, and jack pine in the LAMF ecosystem, respectively. The total carbon density of forested land was estimated at 154.4 tC ha⁻¹ with the proportion of 4:6 for total biomass and soil. The analysis of net carbon balance of ecosystem suggested that the LAMF forest ecosystem was acting as a carbon sink with an allowable harvest in the 1990s.     

Cells of the connective tissue differentiate and migrate into pollen sacs

In angiosperms, archesporial cells in the anther primordium undergo meiosis to form haploid pollen, the sole occupants of anther sacs. Anther sacs are held together by a matrix of parenchyma cells, the connective tissue. Cells of the connective tissue are not known to differentiate. We report the differentiation of parenchyma cells in the connective tissue of two Gordonia species into pollen-like structures (described as pseudopollen), which migrate into the anther sacs before dehiscence. Pollen and pseudopollen were distinguishable by morphology and staining. Pollen were tricolpate to spherical while pseudopollen were less rigid and transparent with a ribbed surface. Both types were different in size, shape, staining and surface architecture. The ratio of the number of pseudopollen to pollen was 1:3. During ontogeny in the connective tissue, neither cell division nor tetrad formation was observed and hence pseudopollen were presumed to be diploid. Only normal pollen germinated on a germination medium. Fixed preparations in time seemed to indicate that pseudopollen migrate from the connective tissue into the anther sac.     

The functionality of female reciprocal calls in the Iberian midwife toad (<Emphasis Type="Italic">Alytes cisternasii</Emphasis>): female-female acoustic competition?

Female midwife toads (genus Alytes) emit highly variable reciprocal calls of unclear function prior to and during courtship. In some species, female-female competition, expressed as physical fighting, has been reported. Males of Majorcan midwife toads (Alytes muletensis) show phonotactic response to female calls, and females of Iberian midwife toads (Alytes cisternasii) respond differently according to the male call characteristics. In this study, I test the hypothesis of female-female acoustic competition as an additional function of female reciprocal calls. Playback tests indicate that female calls are not clearly involved in female acoustic competition in the Iberian midwife toad, therefore female calls could be directed at males rather than towards competitive females.     

Gamergates in the myrmicine genus <Emphasis Type="Italic">Metapone</Emphasis> (Hymenoptera:Formicidae)

Gamergates (i.e. mated reproductive workers) are mostly known from ant species within the Ponerinae. We report here the discovery of gamergates in two species of the subfamily Myrmicinae. Until now, mated reproductive females in colonies of myrmicine species have been considered morphologically distinct from the worker caste. However, in two species of the myrmicine Metapone (Metapone madagascarica and a newly discovered, not yet described Metapone species) all workers have six ovarioles and a spermatheca; and some of them are mated. There are no morphological differences between mated and non-mated workers. Field observations and laboratory studies indicate that colonies of the Metapone species can reproduce with gamergates only.     

Vocal acrobatics in a Chinese frog, <Emphasis Type="Italic">Amolops tormotus</Emphasis>

Although amphibians are highly vocal, they generally emit only a limited number of acoustic communication signals. We report here the extraordinarily rich vocal repertoire of Amolops tormotus, a ranid species in China. These frogs produce countless vocalizations, some of which share features of birdsong or primate calls, e.g., ultrasonic frequency components, multiple upward and downward FM sweeps, and sudden onset and offset of selective harmonic components within a call note. Frame-by-frame video analysis of the frog's calling behavior suggests the presence of two pairs of vocal sacs that may contribute to the remarkable call-note complexity in this species. Electronic supplementary material to this paper can be obtained by using the Springer LINK server located at http://dx.doi.org/10.1007/s00114-002-0335-x.     

Inventory of emissions from major air pollutant categories in Turkey

In this study, an inventory of air pollutant emission estimates from major air polluting sources in Turkey for period between 1985 and 2005 with 5-year intervals were estimated. Inventory covers anthropogenic sources of five major air pollutants of particulate matter, sulfur dioxide, carbon monoxide, nitrogen oxides and non-methane volatile organic compounds. Their break-down with respect to main activity sectors were shown and their distribution by the largest industrial source categories were worked out as annual estimates. This inventory and its analysis point to serious environmental implications of air pollutants and a need to develop a policy plan for reducing these emissions.     

Practical environmental monitoring of major construction projects

Independent teams undertook environmental monitoring of particular concentrations of major construction projects forming part of Hong Kong’s U.S. $20 billion airport infrastructure programme located in dense urban areas. The team combination of environmental specialists with experienced civil engineers enabled pragmatic mitigation measures to be developed and accepted by the construction personnel with the result that potentially significant adverse impacts were averted. The authors discuss the mechanism and success of this innovative approach.